1291 pET30b-TAT-NLS-sso7d
Cloning of 1291 pET30b-TAT-NLS-sso7d
[edit]Purpose: Cloning of 1291 pET30b-TAT-NLS-sso7d
Date: July 31, 2012
Notebook:
Page:
Clone ID: 1291
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: July 31, 2012
Gene synthesis of PCR product. Expected size ~100 bp
See lane 2
Cut out and purify band, digest with RE Nde1 + Kpn1 (lane 1) with vector 368 pET30b-sso7d-KX (lane 2)
Cut out bands, purify and ligate over night
Date: August 6, 2012
MiniPrep screening came back all negative, started all over again
Gene synthesis of PCR product. Expected size ~100 bp
See lane 2
Cut out and purify band, digest with RE Nde1 + Kpn1 (lane 1) with vector 368 pET30b-sso7d-KX (lane 2)
Cut out bands, purify and ligate over night
Colony Screening
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Digested MiniPrep with RE Nde1 + Kpn1, no inserts
Date: August 9, 2012
Digested MIniPrep with RE Nde1 and Kpn1, faint inserts at expected ~100 bp
1, 2, 3, 5 and 6 may be correct
Result intepretation/Summary
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Conclusion
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