1305-pCMV5-Fc(3C)-TagRFP

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Cloning of 1305-pCMV5-Fc(3C)-TagRFP

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Purpose: Cloning of 1305-pCMV5-Fc(3C)-TagRFP

Date: 2012-8-14---2012-8-16

Notebook:0

Page:

Clone ID:1305

Experimenters: yingying

Results

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cloning

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 lane 3
 lane 4

 PCR product
 pCMV5-Fc(3C) 1262
enzymes
   Not1 + Nhe1



Colony Screening

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Date: 2012-8-16

Make miniprep and digest with Not1 + Nhe1

clone 1,2,4,5,6 may be right,need to sequence

Result intepretation/Summary

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Conclusion

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