110 pLVX-TagGFP
110: pLVX-TagGFP
8/11/2012
1 to 6 digest with Nde1 + Xba1
1* digest with Nde1 + Xho1
Nde1--CMV--Xho1--EcoR1--BamH1--TagGFP--Xba1
Cloning of 110 pLVX-TagGFP
[edit]Purpose: Cloning of 110 pLVX-TagGFP
Date: August 15, 2012
Notebook:
Page:
Clone ID: 110
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: August 15, 2012
subcloning TO 153 pLVX-Plus
digest 109 pET28b-TagGFP with BamH1 + Xba1 -->> TagRFP insert (lane 1)
Digest vector 153 pLVX-Plus with BamH1 + Xba1 -->> vector
Lost vector, digest vector again with RE BamH1 and Xba1, ligate together and transform
Colony Screening
[edit]Date: August 17, 2012
Digested MiniPrep with RE BamH1 and Xba1
Digested samples 1, 2, 3 in lanes 1, 2, 3 respectively with RE EcoR1 and Xba1
Result intepretation/Summary
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Conclusion
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