1207-pDB.His.MBP-(3C)
Cloning of 1207-pDB.His.MBP-(3C)
[edit]Purpose: Cloning of 1207-pDB.His.MBP-(3C)
Date: 2012-6-29----
Notebook:
Page:
Clone ID: 1207
Experimenters:yingying lu
Cloning
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lane 1: digest 360 pDB.His.MBP with Nde1 + BamH1,
ligate to annealled oligos
MBP-3C-T
T Agc CTG GAA GTT CTG TTC CAG GGG CCC CAT ATG CTG CAG gct agc g
MBP-3C-B
gatccgctagcCTGCAGCATATGGGGCCCCTGGAACAGAACTTCCAGgc
Date: August 17, 2012
lane 1: digest 360 pDB.His.MBP with Nde1 + BamH1,
ligate to annealled oligos
MBP-3C-T
T Agc CTG GAA GTT CTG TTC CAG GGG CCC CAT ATG CTG CAG gct agc g
MBP-3C-B
gatccgctagcCTGCAGCATATGGGGCCCCTGGAACAGAACTTCCAGgc
Colony Screening
[edit]Date: August 20, 2012
Digest with Pst + Xba1--->1.2 kb
Clones 1, 2, 3, 4, 5 and 6 may be correct
Result intepretation/Summary
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Conclusion
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