1342 pDB.His.MBP-(3C)-p53 (1-363)
Cloning of 1342 pDB.His.MBP-(3C)-p53 (1-363)
[edit]Purpose: Cloning of 1342 pDB.His.MBP-(3C)-p53 (1-363)
Date: August 24, 2012
Notebook:
Page:
Clone ID: 1342
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: August 24, 2012
PCR from 80 pLex-p53wt
P53-Nhe1-F
atcgac GCTAGC ATGGAGGAGCCGCAGTCAGATC
P53-EcoR1-R2
(AAGGAGCCAGGGGGGAGCAGGTGA gaattc atcgac)
gtcgatgaattcTCACCTGCTCCCCCCTGGCTCCTT
See lane 2
Digested insert (lane 4) and vector 1207 pDB.His.MBP-(3C) (lane 5) with RE Nhe1 and EcoR1
Cut out bands, ligate together, transform
Colony Screening
[edit]2012-8-27
Nhe1 and EcoR1
Result intepretation/Summary
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Conclusion
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