1343 pDB.His.MBP-(3C)-p53 (1-342)
Cloning of 1343 pDB.His.MBP-(3C)-p53 (1-342)
[edit]Purpose: Cloning of 1343 pDB.His.MBP-(3C)-p53 (1-342)
Date: August 24, 2012
Notebook:
Page:
Clone ID: 1343
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: August 24, 2012
PCR from 80 pLex-p53wt
P53-Nhe1-F
atcgac GCTAGC ATGGAGGAGCCGCAGTCAGATC
1-342
P53-EcoR1-R3
(GAGCGCTTCGAGATGTTCCGATGA gaattc atcgac)
gtcgatgaattcTCATCGGAACATCTCGAAGCGCTC
See lane 1
Digested DNA (lane 1) with RE Nhe1 + EcoR1
Digested vector (lane 1) with RE Nhe1 + EcoR1
Cut out bands, ligate together and transform
Colony Screening
[edit]2012-8-27
Nhe1 and EcoR1
Result intepretation/Summary
[edit]
Conclusion
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