1344 pDB.His.MBP-(3C)-PLK1wt

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Cloning of 1344 pDB.His.MBP-(3C)-PLK1wt

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Purpose: Cloning of 1344 pDB.His.MBP-(3C)-PLK1wt

Date: August 24, 2012

Notebook: 

Page:

Clone ID: 1344

Experimenters: Jennifer Truong

Results

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Cloning

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Date: August 24, 2012

Cloning Method

PCR from 73 pLex-PLK1-wt
PLK1-Nhe1-F
cagtacGCTAGCATGAGTGCTGCAGTGACTGC
PLK1-EcoR1-R
(CAACCGTCTCAAGGCCTCCTAAgaattc ctagac)
gtctaggaattcTTAGGAGGCCTTGAGACGGTT

See lane 4

Digested DNA (see lane 4) with RE Nhe1 + EcoR1

Digested vector 1207 (see lane 3) with RE Nhe1 + EcoR1

Cut out bands, purify, ligate together, transform

Colony Screening

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2012-8-27

Nhe1 and EcoR1


Result intepretation/Summary

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Conclusion

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