1238 pCDNA3.1-DEST
Cloning of pCDNA3.1-DEST
[edit]Purpose: Cloning of pCDNA3.1-DEST
Date: 06/12/2012
Notebook:
Page:
Clone ID: 1238
Experimenters: Paul Xu
Cloning
[edit]
Colony Screening
[edit]Date: 06/12/2012
Digested with Kpn1 + Xho1, inconclusive results (supposed to be ~1.7 kb)
6/15/2012
digest with Kpn1 + Xho1
Result intepretation/Summary
[edit]
Conclusion
[edit]
Purpose: Cloning of pCDNA3.1-DEST
Date: 06/20/2012
Notebook:
Page:
Clone ID: 1238
Experimenters: yingying lu
| lane 3 |
lane 4 | |
| 374 pAd5-CMV-Dest-C6XHis |
1226 pCDNA3.1 | |
| enzymes |
Kpn1 + Xho1 | |
2012-6-25
digest detect:Kpn1 + Xho1
2012-6-26
1238-clone5 digest detect
2012-6-29
band 1
1238-5 digest :BamH1
gateway reaction
2012-9-5
