1341 pDB.His.MBP-(3C)-p53wt
Cloning of 1341 pDB.His.MBP-(3C)-p53wt
[edit]Purpose: Cloning of 1341 pDB.His.MBP-(3C)-p53wt
Date: August 24, 2012
Notebook:
Page:
Clone ID: 1341
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: August 24, 2012
PCR from 80 pLex-p53wt
P53-Nhe1-F
atcgac GCTAGC ATGGAGGAGCCGCAGTCAGATC
P53-EcoR1-R1
(GAAGGGCCTGACTCAGACTGA gaattc atcgac)
gtcgatgaattcTCAGTCTGAGTCAGGCCCTTC
See lane 3
Digested DNA (lane 3) with RE Nhe1and EcoR1
Digested vector 1207 with RE Nhe1 and EcoR1 (see lane 2)
Cut out bands, purifiy and ligate together, transform
Colony Screening
[edit]
Result intepretation/Summary
[edit]
Conclusion
[edit]
Cloning of 1341 pDB.His.MBP-(3C)-p53wt
[edit]Purpose: Cloning of 1341 pDB.His.MBP-(3C)-p53wt
Date: August 30, 2012
Notebook:
Page:
Clone ID: 1341
Experimenters: yingying lu
Results
[edit]Cloning
[edit]Date: August 30, 2012
PCR from 80 pLex-p53wt
P53-Nhe1-F
atcgac GCTAGC ATGGAGGAGCCGCAGTCAGATC
P53-EcoR1-R1
(GAAGGGCCTGACTCAGACTGA gaattc atcgac)
gtcgatgaattcTCAGTCTGAGTCAGGCCCTTC
Digested DNA (lane ) with RE Nhe1and EcoR1
Digested vector 1207(lane4) with Nhe1 and EcoR1
Cut out bands, purifiy and ligate together, transform
Colony Screening
[edit]2012-9-5
Result intepretation/Summary
[edit]sequence result: not the right colony
Conclusion
[edit]
Cloning of 1341 pDB.His.MBP-(3C)-p53wt
[edit]Purpose: Cloning of 1341 pDB.His.MBP-(3C)-p53wt
Date: 2012-9-12---2012-9-14
Notebook:
Page:
Clone ID: 1341
Experimenters: yingying lu
Results
[edit]Cloning
[edit]Date: August 30, 2012
PCR from 80 pLex-p53wt
P53-Nhe1-F
atcgac GCTAGC ATGGAGGAGCCGCAGTCAGATC
P53-EcoR1-R1
(GAAGGGCCTGACTCAGACTGA gaattc atcgac)
gtcgatgaattcTCAGTCTGAGTCAGGCCCTTC
Digested DNA (lane ) with RE Nhe1and EcoR1
Digested vector 1342 (lane4) with Nhe1 and EcoR1
Cut out bands, purifiy and ligate together, transform
Colony Screening
[edit]2012-9-14
mini prep and digest :Nhe1+EcoR1
Result intepretation/Summary
[edit]
Conclusion
[edit]