1353 pCMV5-Fc(3C)-RFP-WPRE

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Cloning of 1353 pCMV5-Fc(3C)-RFP-WPRE

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Purpose: Cloning of 1353 pCMV5-Fc(3C)-RFP-WPRE

Date: August 21, 2012

Notebook: 

Page:

Clone ID: 1353

Experimenters: Jennifer Truong

Results

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Cloning

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Date: August 21, 2012

Subclone RFP from 1305 pCMV5-Fc(3C)-TagRFP using Nhe1 + Not1


Digested insert 1305 pCMV5-Fc(3C)-TagRFP (lane 1) and vector 1336 (lane 2) with RE Nhe1 + Not1

Cut out bands, purify and ligate together, transform

Colony Screening

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2012-8-27

Nhe1 + Not1

2012-9-14


Result intepretation/Summary

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Conclusion

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