PKA ELISA 4-6-2011

Notebook Zhen Tao #10

Page 2096

All samples are diluted 1/10000 with blocking solution (1%BSA, 10%glycerol in TBS).

Control: only 2nd antibody (anti-human) was used;
sample 31~40 are normal people serum;
sample 447667~447669: prostate cancer people serum;
sample 447670~447672: colon cancer people serum;
sample 447673~447674: breast cancer people serum.

Samples	OD450-1	OD450-2	OD450-3	average	average of normal serum sample
control	0.019	0.017	0.016	0.017	0.036
31	0.031	0.033	0.034	0.033
32	0.044	0.043	0.043	0.043
33	0.037	0.037	0.045	0.040
34	0.029	0.035	0.046	0.037
35	0.043	0.042	0.040	0.042
39	0.032	0.033	0.032	0.032
40	0.027	0.024	0.026	0.026
447667	0.108	0.094	0.107	0.103
447668	0.087	0.077	0.082	0.082
447669	0.039	0.036	0.030	0.035
447670	0.051	0.045	0.042	0.046
447471	0.030	0.031	0.027	0.029
447672	0.035	0.035	0.039	0.036
447673	0.058	0.063	0.054	0.058
447674	0.023	0.025	0.026	0.025
positive control	0.345	0.339	0.342	0.342

--Ztao 14:26, 6 April 2011 (EST)