1353-1305-1369 transfection on 09-11-2012

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No.
 ID
 Plasmid Name                              
conc.(ug/ul) TR (transfection reagent
Color
1
 1353
 pCMV5-Fc(3C)-RFP-WPRE
 2.0 PEI
 no
2
 1305
 pCMV5-Fc(3C)-TagRFP
 1.0 PEI
 no
3
 1369
 pCMV5-Fc-(3c)-TagRFP-WPRE-oriP
 2.8 PEI
 no

12 well plate transfection:
use 1ug DNA plasmid and 3 ul PEI TR and diluted into 50 ul DMEM medium . There is no RFP in clone 1353,1305 and 1369 .





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