1287-pFastbac1-VVDP
Cloning of 1287-pFastbac1-VVDP
[edit]Purpose: Cloning of 1287-pFastbac1-VVDP
Date: 2012-8-21---2012-8-24
Notebook:0
Page:
Clone ID:1287
Experimenters: yingying
Results
[edit]cloning
[edit]template: vaccinia virus genomic DNA
VVDP-BamH1-6H-F
VVDP-Xho1-R
digest PCR product with BamH1 + Xho1, clone into BamH1 + Xho1, 292 pFastBac1
Colony Screening
[edit]Date: 2012-08-24
Make miniprep and digest with BamH1 + Xho1
Digested MiniPrep with RE Nde1
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
Cloning of 1287-pFastbac1-VVDP
[edit]Purpose: Cloning of 1287-pFastbac1-VVDP
Date: 2012-8-29---2012-8-31
Notebook:0
Page:
Clone ID:1287
Experimenters: yingying
Results
[edit]cloning
[edit]template: vaccinia virus genomic DNA
VVDP-BamH1-6H-F
VVDP-Xho1-R
digest PCR product with BamH1 + Xho1, clone into BamH1 + Xho1, 292 pFastBac1
Colony Screening
[edit]Date: 2012-08-31
Make miniprep and digest with BamH1 + Xho1
no insert
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>