1376-pCMV5-TPA-(3c)-Fc-WPRE-OriP
Cloning of 1376-pCMV5-TPA-(3c)-Fc-WPRE-OriP
[edit]Purpose: Cloning of 1376-pCMV5-TPA-(3c)-Fc-WPRE-OriP
Date: 2012-9-26---2012-9-29
Notebook:0
Page:
Clone ID:1376
Experimenters: yingying
Results
[edit]cloning
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gene synthesis of ss and amplification of Fc from 1361 pCDM8-like-nSEBOV-delta-thr-Fc
TPA-BamH1-F1, 1 ul of 10 uM
TPA-BamH1-F2 , 0.5 ul of 1 uM
TPA-BamH1-F3, 0.5 ul of 1 uM
TPA-BamH1-F4, 0.5 ul of 1 uM
Fc-6His-Eag1-R, 1 ul of 10 uM
| digest PCR product with BamH1 + Eag1, digest vector 1369 pCMV5-Fc-(3c)-TagRFP-WPRE-oriP with BamH1+ Eag1 |
Colony Screening
[edit]Date: 2012/9/29
1376 digest with BamH1 + Eag1, size is about 1.5 kb
Result intepretation/Summary
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Conclusion
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