1414 pCDNA3.4-Rabbit-IgG Light Chain

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Cloning of 1414 pCDNA3.4-Rabbit-IgG Light Chain

Purpose: Cloning of 1414 pCDNA3.4-Rabbit-IgG Light Chain

Date: 2012-10-3 ~2012-10-5

Notebook:

Page:

Clone ID: 1414

Experimenters: yingying

Results

Cloning

Date:10-3-2012

Digest 1 ug plasmid DNA 1410 pCDNA3.4-Rabbit-IgG H+L with Xba1 + Age1

cut out H & L, also vector. Ligate and transform DH5alpha

Colony Screening

2012-10-5

mini prep and digest with Xba1 + Age1

Result intepretation/Summary

Conclusion

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Cloning