1386 pCMV5-TPA-RIPK3-(3c)-Fc-WPRE-oriP
Cloning of 1386 pCMV5-TPA-RIPK3-(3c)-Fc-WPRE-oriP
[edit]Purpose: Cloning of 1386 pCMV5-TPA-RIPK3-(3c)-Fc-WPRE-oriP
Date: 2012-9-29 ~
Notebook:0
Page:
Clone ID: 1396
Experimenters: Paul & Shanyi & Xiuli
Results
[edit]PCR
[edit]2012-9-29
2012/9/30 digestion:
| Lane |
1 |
2 |
3 |
4 |
| Name |
TagGFP |
1369 |
1376 |
TagGFP |
| Digestion |
Nhe1+Not1 |
Nhe1+Not1 |
Nhe1 + Kpn1 |
Nhe1 + Kpn1 |
Colony Screening
[edit]Date: 10/7/2012
clone #3 & 4 may be ok
10-08-2012
Re-screening colony with PCR methods. #7 #8 in 1386 below is the same clone #3 and #4 above miniprep.
1, 3, 4, 5, 6 looks right size. but #7 and #8 didn't work.( it's positive clone #3 and #4 in miniprep screening.
Result intepretation/Summary
[edit]