1411-pCMV5-IgK-Flag-6His-Fc(3c)-Dest-WPRE-oriP
Cloning of 1411-pCMV5-IgK-Flag-6His-Fc(3c)-Dest-WPRE-oriP
[edit]Purpose: Cloning of 1411-pCMV5-IgK-Flag-6His-Fc(3c)-Dest-WPRE-oriP
Date: 2012-10-9---2012-10-12
Notebook:0
Page:
Clone ID:1411
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR Fc from 1361 pCDM8-like-nSEBOV-delta-thr-Fc
Fc-3C-Nhe1-R
1 ul of 10 uM Fc-3C-Nhe1-R
1 ul of 10 uM mIgK-BamH1-F1
1 ul of 1 uM mIgK-BamH1-F2
1 ul of 1 uM mIgK-BamH1-F3
1 ul of 1 uM mIgK-BamH1-F4
Digest PCR product (lane7) with BamH1 + Nhe1, ligate with BamH1 + Nhe1 digested vector 1349 pCMV5-Fc-(3c)-Dest-WPRE-oriP (lane8)
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Colony Screening
[edit]Date: 2012-10-12
Make miniprep and digest with BamH1 + Nhe1
need to sequence
Result intepretation/Summary
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Conclusion
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