1352-pET28b-Cre
Cloning of 1352-pET28b-Cre
[edit]Purpose: Cloning of 1352-pET28b-Cre
Date: August 30, 2012---2012-9-4
Notebook:
Page:
Clone ID: 1352
Experimenters: yingying lu
Results
[edit]Cloning
[edit]Date: August 30, 2012
PCR using 298 pTriEx-HTNC as template
Cre-Nde1-F
cagtca catatg tcc aat tta ctg acc gta cac
Cre-Xho1-R
(gaagatggcgattagctcgag ctgaca)
tgtcagctcgagctaatcgccatcttc
Colony Screening
[edit]2012-8-31
no colonies
Result intepretation/Summary
[edit]
Conclusion
[edit]
Cloning of 1352-pET28b-Cre
[edit]Purpose: Cloning of 1352-pET28b-Cre
Date: 2012-10-10----2012-10-15
Notebook:
Page:
Clone ID: 1352
Experimenters: yingying lu
Results
[edit]Cloning
[edit]Date: , 2012
PCR using 298 pTriEx-HTNC as template
Cre-Nde1-F
cagtca catatg tcc aat tta ctg acc gta cac
Cre-Xho1-R
(gaagatggcgattagctcgag ctgaca)
tgtcagctcgagctaatcgccatcttc
| lane 12 |
lane 13 | |
| PCR product |
1026- pET28b-LambdaExo | |
| enzymes |
Nde1+Xho1 | |
Colony Screening
[edit]2012-10-15
clone 2,3,4 need to sequence
Result intepretation/Summary
[edit]
Conclusion
[edit]