1425-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-Gluc

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Cloning of 1425-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-Gluc

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Purpose: Cloning of 1425-pLVX-Kozak-loxP-TurboRFP-ODC-loxP-Gluc

Date: 2012-10-23---2012-10-25

Notebook:0

Page:

Clone ID:1425

Experimenters: yingying

Results

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cloning

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Template:1271 pLVX-loxP-TurboRFP-ODC-loxP-TagRFP

LoxP-TurboRFP-BamH1-F1+LoxP-TurboRFP-BamH1-F2+LoxP-TurboRFP-BamH1-R




 lane1
 lane2

 PCR product
 1079 pLVX-loxP-sfGFP-loxP-Gluc
enzymes
        BamH1 + Xho1



Colony Screening

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Date: 2012-10-25

Make miniprep and digest with BamH1 + Xho1

clone 1,2,3,4,5,6 need to sequence

Result intepretation/Summary

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Conclusion

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