1382-1406 Purification on 11-7-2012
1382-1406 purification on 11-07-2012 by Xiuli
Cell Viability: 1386 has less cells and poor viability,
Collect 30 ml Serum-free medium from each flasks (5) and and 1 plate (1382) 20 ml and centriguged at 12000rpm for 15 mins.
Incubate supernanats with protein A beads, washed with PBS and elute with 0.2 mM Glycine (pH=2.0), elution volume:300 ul
Predicted expressed protein size
| 1382 | 1383 | 1385 | 1386 | 1406 |
| 110KD | 48-54KD | 79KD | 60KD | 61KD
|
Load 20-35 ul on the 4-20% SDS gel
| 1382 | 1383 | 1385 | 1386 | 1406 | 1386 | |
| Lane |
1,2,3 |
4,5,6 | 7,8,9, | 10,11,12 | 13,14,15, | 16
|
The protein size is not the predicted size except 1383.