1095-pET28b-T7Exo
Cloning of 1095-pET28b-T7Exo
[edit]Purpose: Cloning of 1095-pET28b-T7Exo
Date: 2012-4-13---2012-4-19
Notebook:0
Page:
Clone ID:1095
Experimenters: yingying
Results
[edit]cloning
[edit]
template: PCR using T7 Phage DNA
T7_Exo_Nde1-F
cacagg cat atg gcc atggcacttcttgaccttaaac
T7_exo-Xho1_R
(gagattta cctgtggaga ccgtag ctcgag actagc)
gctagtctcgagctacggtctccacaggtaaatctc
| band 3 |
band 4 | |
| PCR product |
pET28b | |
| enzymes |
Nde1+Xho1 | |
Colony Screening
[edit]Date: 2012-4-19
Make miniprep and digest with Nde1+Xho1
clone 1 3 4 7 may be right, need to sequence
Result intepretation/Summary
[edit]Conclusion
[edit]Cloning of 1095-pET28b-T7Exo
[edit]Purpose: Cloning of 1095-pET28b-T7Exo
Date: 2012-10-19---2012-10-26
Notebook:0
Page:
Clone ID:1095
Experimenters: yingying
Results
[edit]cloning
[edit]
template: PCR using T7 Phage DNA
T7_Exo_Nde1-F
cacagg cat atg gcc atggcacttcttgaccttaaac
T7_exo-Xho1_R
(gagattta cctgtggaga ccgtag ctcgag actagc)
gctagtctcgagctacggtctccacaggtaaatctc
| band 2 |
band | |
| PCR product |
||
| enzymes |
Nde1+Xho1 | |
| lane2 |
||
| 1027,pET28b-LambdaBet |
||
| enzymes |
Nde1+Xho1 |
Colony Screening
[edit]Date: 2012-10-26
Make miniprep and digest with Nde1+Xho1
2012-11-8
PCR Screening: T7_Exo_Nde1-F+T7 term
