Chromatography Basic

linear flow (cm/h) to volumetric flow rate (ml/min)

Volumetric flow rate (ml/min) = Linear flow (cm/h) × column cross-sectional area (cm2)/60=Y * Pi * d² /60*4

Y = linear flow in cm/h
d = column inner diameter in cm
Example:
What is the volumetric flow rate in an XK 16/70 column (i.d. 1.6 cm) when the linear flow is 150 cm/h?
Y = linear flow = 150 cm/h
d = inner diameter of the column = 1.6 cm
Volumetric flow rate = 150 * Pi * 1.6 * 1.6 ml/min / 60 * 4 = 5.03 ml/min

For XK16 column, 60 cm/h =2 ml/min

For Hitrap 1 ml column: ID=0.7 cm,  60 cm/h =0.4 ml/min

For HiTrap 5 ml column and XK16 column: ID=1.6  cm,  60 cm/h =2 ml/min