240-pET28b-T4-Ligase
Cloning of 240-pET28b-T4-Ligase
[edit]Purpose: Cloning of 240-pET28b-T4-Ligase
Date: 2012-10-11---2012-10-15
Notebook:0
Page:
Clone ID:240
Experimenters: yingying
Results
[edit]cloning
[edit]1st PCR:Gene synthesis
Frag 1: 1-14
Frag 2: 15-30
Frag 3: 31-44
2nd PCR
| band 1 |
band 2 | |
| PCR product |
pET28b | |
| enzymes |
Nde1+Xho1 | |
Colony Screening
[edit]Date: 2012-10-15
Make miniprep and digest with Nde1+Xho1
c
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
<br>
<br>
Cloning of 240-pET28b-T4-Ligase
[edit]Purpose: Cloning of 240-pET28b-T4-Ligase
Date: 2012-10-19---2012-10-21
Notebook:0
Page:
Clone ID:240
Experimenters: yingying
Results
[edit]cloning
[edit]2nd PCR:2012-10-19
2012-10-23
| lane1 |
||
| PCR product |
||
| enzymes |
Nde1+Xho1 |
2012-10-24
| lane1 | ||
| 1026,pET28b-LambdaExo | ||
| enzymes |
Nde1+Xho1 | |
Colony Screening
[edit]Date: 2012-10-21
Make miniprep and digest with Nde1+Xho1
clone 5 need to sequence
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
<br>
<br>
Cloning of 240-pET28b-T4-Ligase
[edit]Purpose: Cloning of 240-pET28b-T4-Ligase
Date: 2012-11-20---2012-10-28
Notebook:0
Page:
Clone ID:240
Experimenters: yingying
Results
[edit]cloning
[edit]2nd PCR:2012-10-19
2012-11-20
| lane 1 |
lane2 | |
| PCR product |
1027,pET28b-Lambda | |
| enzymes |
Nde1+Xho1 | |
Colony Screening
[edit]Date: 2012-11-27
Make miniprep and digest with Nde1+Xho1
PCR screening:T4 1-1+T7tem
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
<br>
<br>