PKA alpha purification Dec 2012

1. Nov 2012

Purify using Ni column

2. Nov 29 2012

From 8 L: Total wet cell: 30 g

Induction: 1 ml of 20% L-arabinose, 37oC o/n

Purify using 15 ml Ni column, elute protein with 300 mM Imidazole, concentrate using Pierce 10k spin column,  then load to gel filtration column Bio-Rad BioSil TSK-250. Program is 47. injection: 5 ml, 2.5 ml/min, 4 ml per fraction.

Fraction	Protein Conc mg/ml
34	0.2
35	2.9
36	2.86
37	2.88
38	1.62
39	0.53
40	0.28

Store protein at -80 oC