1467-pAd5-PhiC31
Cloning of 1467-pAd5-PhiC31
[edit]Purpose: Cloning of 1467-pAd5-PhiC31
Date: 2012-11-30---2012-12-5
Notebook:0
Page:
Clone ID:1467
Experimenters: yingying
Results
[edit]cloning
[edit]PCR using Q5 and these primers:
phic31-Kpn1-EcoRV-F
ctacga GGTACC GATATC GCCGCCACC atggatacctacgccggagcc
phic31-BamH1-Xho1-R
(ctaagaaaaagcggaaagtgtga GGATCC ctcgag cgacca)
tggtcgctcgagGGATCCtcacactttccgctttttcttag
template: 1112 pPGKPhiC31obpA
| band 1 |
band 2 | |
| PCR product |
280 pAd5-CMV-MCS | |
| enzymes |
Kpn1 + Xho1 | |
Colony Screening
[edit]Date: 2012-12-5
Make miniprep and digest with Kpn1 + Xho1
c
Result intepretation/Summary
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Conclusion
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