1460 pAd5-LoxP-MCS-IRES-EGFP

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Cloning of 1460 pAd5-LoxP-MCS-IRES-EGFP

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Purpose: Cloning of 1460 pAd5-LoxP-MCS-IRES-EGFP

Date: 2012-11-21-- Notebook:0

Page:

Clone ID:1460

Experimenters: SHANYI

Results

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cloning

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digest 533 pLVX-Cre-IRES-EGFP with Xho1 +Mlu1--> IRES-GFP insert digest 1459 pAd5-LoxP-MCS with Sal1 +Mlu1--> vector

Colony Screening

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Date: 2012-11-


digestion with (EcoR1 + Mlu1) (note: Sal1 is gone) digestion