Aldolase-Expression

Expression of MBP-Aldolase and MBP-VP16 in BL21 (DE3)

MW: 10(bottom), 17, 28(red), 36, 55, 72(red), 95, 130, 250.

1, His-SM2

2, pDB.His.MBP-Aldolase, after purification on Ni and Maltose columns

3, FT, flow through, Maltose column

4, pDB.His.MBP-VP16, uninduced BL21(DE3), eluate from Ni beads.

5, pDB.His.MBP-VP16, IPTG induced BL21(DE3), clone 1, eluate from Ni beads.

6, pDB.His.MBP-VP16, IPTG induced BL21(DE3), clone 2, eluate from Ni beads.

7, pET28b-VP16, uninduced BL21(DE3), eluate from Ni beads.

8, pET28b-VP16, IPTG induced BL21(DE3), clone 1, eluate from Ni beads.

9, pET28b-VP16, IPTG induced BL21(DE3), clone 2, eluate from Ni beads.

Expression of MBP-Aldolase and MBP-VP16 in BL21 (DE3)

MW: 10(bottom), 17, 28(red), 36, 55, 72(red), 95, 130, 2

1, pDB.His.MBP-Aldolase after Ni and Maltose columns

2, pDB.His.MBP-VP16, flow through after Ni and Maltose columns

3, pDB.His.MBP-Vp16, after Ni and maltose columns

4, pDB.His.MBP-Vp16, after Ni and maltose columns

5, Superfolder GFP

6, Superfolder GFP-TPA fusion (forgot to add protein sample).

7, G-Luciferase

8, Insuin

9, Infared Fluorescent protein.

--Paul.xu 15:42, 5 November 2010 (EST)

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