1500 pT7A3-T3-ligase

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Cloning of 1500 pT7A3-T3-ligase

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Purpose: Cloning of 1500 pT7A3-T3-ligase

Date: 2012-12-3--2012-12-5

Notebook: 0

Page:

Clone ID:1500

Experimenters: yingying

Results

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subcloning

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lane 5
lane 6

1096 pET28b-T3-Ligase
1279 pT7A3
enzymes
      Nde1 +Xho1


Colony Screening

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Date: 2012-12-5

mini prep and diges with Nde1 +Xho1