1143-1151-1186-screening
Data:2012-11-15--2012-11-16
Exprimenter: Xiuli
[edit]
| 1143 | p8H.T7.Exo-1 |
| 1151 | pET30b-sso7d-ToughTag |
| 1155 | pET28b-Ara-S1 |
| 1156 | p8H-VentExo |
| 1186 | pDB.His.MBP-Xis |
4 ml sample each , 1143, 1151, 1155,1156,1186 induce with 0.5mM IPTG at 37 oC for overnight. Supernants were loaded on the gel.
Supernant:
| 1143 |
1151 |
1186 |
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| p8H.T7.Exo-1 |
pET30b-sso7d-ToughTag |
pDB.His.MBP-Xis |
|||||||||
| lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 |
| IPTG | 0 | 0 | 0.5 | 0.5 | 0 | 0 | 0.5 | 0.5 | 0 | 0.5 | 0 |
| lane | C1 | C2 | E1 | E2 | C1 | C2 | E1 | E2 | C1 | E1 | MBP |
Supernant:
| 1155 |
1156 |
1186 | |||||||||
| pET28b-Ara-S1 |
p8H-VentExo |
pDB.His.MBP-Xis | |||||||||
| lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 19 | 10 | 11 |
| IPTG | 0 | 0 | 0 | 0.5 | 0.5 | 0 | 0 | 0.5 | 0.5 | 0 | 0.5 |
| lane | MBP | C1 | C1 | E1 | E2 | C1 | C2 | E1 | E2 | C2 | E2 |
1156 and 1186 are expressed .