1550 pT7A3-MBP-(3C)-T4-DNA-Ligase
Cloning of 1550 pT7A3-MBP-(3C)-T4-DNA-Ligase
[edit]Purpose: Cloning of 1550 pT7A3-MBP-(3C)-T4-DNA-Ligase
Date: December 27, 2012
Notebook:
Page:
Clone ID: 1550
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: December 26, 2012
Subclone T4 DNA ligase from 1483 pT7A3-T4_DNA_Ligase using Nde1 + Xho1
Vector Name 1514 pT7A3-MBP-(3C)
Ligate lane 3 (insert 1483) together with lane 4 vector (1514)
Transform
Colony Screening
[edit]Date: December 31, 2012
Picked colonies from plate, grew up 6 samples, Miniprepped
Digested plasmids with Xho1 and Nde1
See lanes 7-12, samples 2-6 look like they have inserts
Submitted lane 12 (sample 6) for sequencing
Result intepretation/Summary
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Conclusion
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