1465 1510 1511 1514 1517

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Data:2012-12-29---2012-12-31

Exprimenter: yingying

3 ml sample each , 1465,1510,1511,1517  induce with 0.5mM IPTG at  37 oC 0/N overnight

1465  pT7A3-MBP-3C
1510 pT7A3-MBP.Native-3C
1511 pT7A3-MBP.Synthetic-3C
1514
 pT7A3-MBP-(3C)
1517
 pT7A3-MBP-(3C)-sfGFP



 1
 2
 3
 4
 5
 6
 7
 8
Clone ID
 1465
 1511
 1510
 1511
 1514
 1517
 1429
 1429control
competent cell
 BL21 Star DE3
 Rosetta2 DE3 plysS
           Nico21 DE3 plysN
 









 conclusion :1517 pT7A3-MBP-(3C)-sfGFP has  expression








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