1495-1496-1497-screening

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Data:2012-12-17---2012-12-20

Exprimenter: yingying

3 ml sample each , 1495,1496,1497  induce with 0.5mM IPTG at  37 oC 0/N overnight

1495 pT7A3-MMLV
1496 pT7A3-DNase1
1497 pT7A3-ToughTaq



lane 1 2 3 4 5 6 7 8
IPTG mM 0.5 0 0.5 0 0.5 0 0.5 0
clone ID
1429 MBP
1495 MMLV
1496 Dnase1
1497 ToughTaq


 conclusion : ToughTaq has low expression (lane7) 70KD