1600-pT7A3-6His-GST-3C

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Cloning of 1600-pT7A3-6His-GST-3C

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Purpose: Cloning of 1600-pT7A3-6His-GST-3C

Date: 2013-1-11---2013-1-14

Notebook:0

Page:

Clone ID:1600

Experimenters: yingying

Results

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subcloning

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lane 3 and lane 4


Subclone 3C from 1420 pDB.His.MBP.3C to  1597 pT7A3-6XHis.GST using Nde1 + Xho1


Colony Screening

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Date: 2013-1-14

Make miniprep and digest with Nde1 + Xho1

Result intepretation/Summary

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Conclusion

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