Signal peptide comparison

Transfection:

5 ul  (100 ~ 200 ng/ul) from miniprep, each well is from individuale clone

24 well plate.

1393: pCMV5-IL2-TagGFP-(3c)-Fc-WPRE-OriP

1394: pCMV5-CST4-TagGFP-Fc(3c)-WPRE-OriP

1395: pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP

1396: pCMV5-hIgG-Hc-TagGFP-Fc(3c)-WPRE-OriP

Nov 2012

Transfect with L2K. 1433 plasmid DNA quality is low, only 20 ng/ul

2012/11/23

11/24/2012

11/20/2012: Transfection using lipofectamine 2000, 1 ug DNA, 3 ul L2K, 12 well plate

Picture on 2012/11/22

Picture on 2012/11/23

11/24/2012

2012/11/25

Signal_seq_comparison_2013_1_24.jpg‎

11/21/2012: Transfection using GeneExpression Max

1 ug DNA, 3 ul GEM, 12 well plate

Picture on 11/22/2012

2012/11/24

2012/11/25

2012/12/12

transfection on 2012/12/11: 1 ug/well, 3 ul GEM, picture taken on 2012/12/12

2012/12/13

2012/12/14

plate

Compare new IL2 signal peptide vs other

12 well plate, 293T cells

Transfection: 1 ug DNA

Transfection reagent: GEM

Date: 2013/1/24

2013 1 25

2013/1/26