1538 pT7A3-MBP-(3C)-T4PNK-Pase-

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Cloning of 1538 pT7A3-MBP-(3C)-T4PNK-Pase-

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Purpose: Cloning of 1538 pT7A3-MBP-(3C)-T4PNK-Pase-

Date: Jan , 2013

Notebook: 

Page:

Clone ID: 1538

Experimenters: Shanyi

Results

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Cloning

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PCR1


2

DIGEST

 PCR

45oc 30s, 65oc 2min, 5cycle, 50oc 30s, 65oc 2min, 24cycle

Colony screen

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digest with NdeI and XhoI

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