1552-pT7A3-MBP-(3C)-T3-DNA-Ligase

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Cloning of 1552-pT7A3-MBP-(3C)-T3-DNA-Ligase

Purpose: Cloning of 1552-pT7A3-MBP-(3C)-T3-DNA-Ligase

Date: 2013-1-28---2013-1-30

Notebook:0

Page:

Clone ID:1552

Experimenters: Shanyi

Results

subcloning

subclone from 1518 pET28b-T3-DNA-Ligase to 1514 pT7A3-MBP-(3C) using Nde1 + Xho1

	band 1	band 2
	1514 pT7A3-MBP-(3C)	1518 pET28b-T3-DNA-Ligase
enzymes	Nde1 + Xho1

Colony Screening

Date: 2013-1-30

Make miniprep and digest with Nde1 + Xho1.

Result intepretation/Summary

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Conclusion

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Cloning