1554-pT7A3-MBP-i3C

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Cloning of 1554-pT7A3-MBP-i3C

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Purpose: Cloning of 1025-pET28b-SM2

Date: 2013-1-10---2013-1-14

Notebook:0

Page:

Clone ID:1554

Experimenters: yingying

Results

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cloning

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 band 1
 band 2

 PCR product
 1429,pT7A3-MBP
enzymes
             Nde1 + Xho1.



Colony Screening

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Date: 2013-1-14

Make miniprep and digest with  Nde1 + Xho1.



Result intepretation/Summary

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Conclusion

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Protein Expression

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1625_pCold-GST-(3c)-KEAP1#Protein_Expression

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