1127 pET30b-T4RNA-Lig2K227Q
Cloning of 1127 pET30b-T4RNA-Lig2K227Q
[edit]Purpose: Cloning of 1127 pET30b-T4RNA-Lig2K227Q
Date: 1, 2013
Notebook:
Page:
Clone ID: 1127
Experimenters: shanyi
Results
[edit]Cloning
[edit]PCR using T4 phage DNA
1: T4RNA-lig-Nde1-F + T4RNAlig-K227Q-R1 using T4 phage DNA as template, not more than 20 cycles
2: T4RNA-lig-Nde1-F + T4RNAlig-K227Q-R2 using PCR product from PCR reaction 1 as template
second round pcr
Clone into pET30b Nde1 + Xho1
ligate and transform, take kan plate,should use amp plate
Colony Screening
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Cloning of 1127 pET30b-T4RNA-Lig2K227Q
[edit]Purpose: Cloning of 1127 pET30b-T4RNA-Lig2K227Q
Date: 2, 2013
Notebook:
Page:
Clone ID: 1127
Experimenters: shanyi
Results
[edit]Cloning
[edit]PCR
1: T4RNA-lig-Nde1-F + T4RNAlig-K227Q-R1 using 1123 as template, not more than 20 cycles
2: T4RNA-lig-Nde1-F + T4RNAlig-K227Q-R2 using PCR product from PCR reaction 1 as template
Clone into pET30b Nde1 + Xho1
ligate and transform,
Colony Screening
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Result intepretation/Summary
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Conclusion
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