1611-pCold-MBP
Cloning of 1611-pCold-MBP
[edit]Purpose: Cloning of 1611-pCold-MBP
Date: 2013-1-10---2013-1-16
Notebook:0
Page:
Clone ID:1611
Experimenters: yingying
Results
[edit]cloning
[edit]
lane 5. digest PCR product with BglII + Nde1
lane 6. Digest vector with BglII + Nde1.
Colony Screening
[edit]Date: 2013-1-16
Make miniprep and digest with BglII + Nde1.
Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 1611-pCold-MBP
[edit]Purpose: Cloning of 1611-pCold-MBP
Date: 2013-1-25---
Notebook:0
Page:
Clone ID:1611
Experimenters: yingying
Results
[edit]cloning
[edit]2012/1/25
pcr1
gene synthesis
pcr2
.pcr1+pcr2
digest PCR product with BamH1 + Nde1
5. Digest vector with BglII + Nde1
Colony Screening
[edit]Date: 2013-
Make miniprep and digest with
Result intepretation/Summary
[edit]Conclusion
[edit]Cloning of 1611-pCold-MBP
[edit]Purpose: Cloning of 1611-pCold-MBP
Date: 2013-2-14---
Notebook:0
Page:
Clone ID:1611
Experimenters: yingying
Results
[edit]cloning
[edit]2012/1/25
pcr1
Method 1: PCR promoter from v19 pET28-cold===
Cold-BamH1-F
Cold-8
gene synthesis
pcr2
.pcr1+pcr2
digest PCR product with BamH1 + Nde1
. Digest vector with BglII + Nde1
Colony Screening
[edit]2013/2/18
Make miniprep and digest with BglII + Nde1
clone 1, 3, 4, 5 may be OK. Send #1 to sequencing