1659-pCold-MBP-(3C)-MCS

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Cloning of 1659-pCold-MBP-(3C)-MCS

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Purpose: Cloning of 1659-pCold-MBP-(3C)-MCS

Date: 2013-2-20----2013-2-20

Notebook:0

Page:

Clone ID:1659

Experimenters: yingying

Results

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cloning

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Digest 1611 pCold-MBP-(3C) with Nde1 + Xho1, gel purify
ligate to annealed oligos Nde1-MCS-Xho1-T + Nde1-MCS-Xho1-B.


Colony Screening

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Date: 2013-2-22

Make miniprep and digest with  Spe1 + BglII




Result intepretation/Summary

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Conclusion

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