Lentivirus-packaging-mix-QC

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1. pGap-Pol: (big one)


EcoR1+ Xho1== 1598 bp
1 XhoI-EcoRI 6764-5168 10555
2 EcoRI-XhoI 5169-67631595

=========
EcoR1 + Nco1
1 EcoRI-NcoI 5169-10137 4969
2 NcoI-EcoRI 373-5168 4796
3 NcoI-NcoI 10138-372 2385


2. pVSVG (small one)

1 EcoRI-NcoI 1186-379 3689
2 NcoI-EcoRI 380-1185 806








PureG: cut out the top band of undigested sigma mix, digest it with EcoR1



--Paul.xu 15:25, 20 May 2011 (EST)

--Paul.xu 17:23, 22 May 2011 (EST)

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