1612-pCold-7His-GST-(E)
Cloning of 1612-pCold-7His-GST-(E)
[edit]Purpose: Cloning of 1612-pCold-7His-GST-(E)
Date: 2013-1-22---2013-1-28
Notebook:0
Page:
Clone ID:1612
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR1:make promoter by gene synthesis
Cold-1 Cold-2 Cold-3 Cold-4 Cold-5 Cold-6 Cold-7 Cold-8
pCold-R
PCR2: PCR GST from pDB.His.GST
7His-GST-F
GST-Enterokinase-Nde1-R
Join PCR products using
Cold-1 + GST-Enterokinase-Nde1-R
Clone into v19 pET28-cold using BglII + Nde1
Colony Screening
[edit]Date: 2013-1-28
Make miniprep and digest with BglII + Nde1
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Result intepretation/Summary
[edit]- &amp;amp;lt;br&amp;amp;gt;
Conclusion
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Cloning of 1612-pCold-7His-GST-(E)
[edit]Purpose: Cloning of 1612-pCold-7His-GST-(E)
Date: 2013-2-1----2013-2-4
Notebook:0
Page:
Clone ID:1612
Experimenters: yingying
Results
[edit]cloning
[edit]pcr1
PCR from v19 pET28-cold using
Cold-BglII-F GGATCGAGATCTGCCGATTAATCATAAATATG
pCold-R atgatgatgatgatgatgatgcactttgtgattcatG
PCR2: PCR GST from pDB.His.GST
7His-GST-F
GST-Enterokinase-Nde1-R
Join PCR products using
Cold-BglII-F + GST-Enterokinase-Nde1-R
Colony Screening
[edit]Date: 2013-1-28
Make miniprep and digest with BglII + Nde1
2013-3-5
lane 7: digest with Nhe1
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Result intepretation/Summary
[edit]- &amp;amp;lt;br&amp;amp;gt;
Conclusion
[edit]&amp;amp;lt;br&amp;amp;gt;
<br>
<br>