Transfection reagents 2012-1-9

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Polymer made on 1/6/2012

Modifier PEI18+Gu: 0.5 g 1H-Pyrazole-1-carboxamidine hydrochloride, 1 g PEI18, 11 ml water

Modifier Gu: 1H-Pyrazole-1-carboxamidine hydrochloride, 100 mg/ml in water.


Code
 Reaction
 Volume of Polymer
 Volume of Modifier
 Water
1PP
 PEI18+ Gu
 1 ml
 50 uL
 200 uL
2PP
 PEI18+ Gu
 1 ml
 100 uL
 150 uL
3PP
 PEI18+ Gu
 1 ml
 150 uL
 100 uL
4PP
 PEI18+ Gu
 1 ml
 200uL
 50 uL
5PP
 PEI18+ Gu
 1 ml
 250 uL
 0 uL
1P
 Gu
 1 ml
 50 uL
 200 uL
2P
 Gu
 1 ml
 100 uL
 150 uL
3P
 Gu
 1 ml
 150uL
 100uL
4P
 Gu
 1 ml
 200uL
 50uL
5P
 Gu
 1 ml
 250uL
 0uL
Control
 -
 1 m

Reaction condition: RT o/n

24 hours after transfection

plate1



plate2



plate3



plate4



48 hours after transfection

plate1


plate2


plate3


plate4



day 3

plate1

plate2

plate3

plate4

day 4

plate1

plate2

plate3

plate4




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