1657-pCold-6His-GST-(E)-(3C)-sfGFP
Cloning of 1657-pCold-6His-GST-(E)-(3C)-sfGFP
[edit]Purpose: Cloning of 1519-pT7A3-T3-DNA-Ligase
Date: 2013-2-14---2013-2-21
Notebook:0
Page:
Clone ID:1657
Experimenters: yingying
Results
[edit]cloning
[edit]PCR sfGFP from 1005 pBad24-Tat-sfGFP using
sfGFP-Kpn1-Nhe1-Sal1-F
sfGFP-HindIII-Spe1-Not1-Xho1-R
Colony Screening
[edit]Date: 2013-2-21
Make miniprep and digest with Nhe1+Xho1
NO sfGFP ,parent vector, 1624 pCold-6His-GST-(E)-(3C) has two Nhe1 sites
Result intepretation/Summary
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Conclusion
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