1548 PiggyBacHR-SMN1-5-3HA

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Cloning of 1548 PiggyBacHR-SMN1-5-3HA

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Purpose: Cloning of 1548 PiggyBacHR-SMN1-5-3HA

Date: 2013-2-11---

Notebook:0

Page:

Clone ID:1548

Experimenters: Shanyi

Results

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PCR

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PCR from 293 genomic DNA



Method: use 1547-2 as vector

digest and ligate together 10x (37 ̊C for 5min + 16 ̊C for 10min) + 50 ̊C for 5min + 80 ̊C for 5min

Colony Screening

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pcr






use plasmid 1234567as template





Result intepretation/Summary

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Conclusion

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