1545-pT7A3-VVCap
Cloning of 1545-pT7A3-VVCap
[edit]Purpose: Cloning of 1545-pT7A3-VVCap
Date: 2013-1-31---2013-2-4
Notebook:0
Page:
Clone ID:1545
Experimenters: yingying
Results
[edit]subcloning
[edit]
lane 1:BglII + Xho1 for 1544 pT7T3-VVCap-L;
lane 2:BamH1 + Xho1 for 1543 pT7T3-VVCap-S
Colony Screening
[edit]Date: 2013-2-4
Make miniprep and digest with Nco1
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]protein screen
[edit]2013/3/28
| lane |
name |
clone ID |
host |
induction |
purification |
MW |
| 1 |
pT7A3-sso7d-Native-ToughTaq |
1542 |
4 ml,
BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
62KD |
| 2 |
pT7A3-VVCap-L |
1544 |
4 ml,
BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
34KD |
| 3 |
pT7A3-VVCap |
1545 |
4 ml,
BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
34KD |
| 4 |
pT7A3-SOD |
1627 |
4 ml,
BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
16KD |
| 5 |
pCold-MBP-SM2 |
1679 |
4 ml,NiCO21(DE3)pLysN, |
0.2 mM IPTG 25 oC o/n |
Ni beads |
74KD |
| 6 |
pCold-GST-wtSM2 |
1680 |
4 ml,NiCO21(DE3)pLysN, |
0.2 mM IPTG 25 oC o/n |
get lysis supernatant to run gel |
94KD |