1588 pT7A3-MBP-(3c)-Exo-VII

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Cloning of ‎1588 pT7A3-MBP-(3c)-Exo-VII

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Purpose: Cloning of 1588 pT7A3-MBP-(3c)-Exo-VII

Date: 2013-3-7---

Notebook:0

Page:

Clone ID:1588

Experimenters: Shanyi

Results

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PCR

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PCR from genomic DNA isolated from Escherichia coli str. K-12 substr. MG1655


Cut out the band, digest PCR products and 1514 pT7A3-MBP-(3C) with Nde1 + Xho1




Colony Screening

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Date: 2013-

Make miniprep and digest withNde1 + Xho1


send 1588-6 to sequence, missing 1 C


Cloning of ‎1588 pEC-Exo-VII

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Purpose: Cloning of 1588 pEC-Exo-VII

Date: 2013-4----

Notebook:0

Page:

Clone ID:1588

Experimenters: Shanyi

Results

[edit]

PCR

[edit]

PCR from genomic DNA isolated from Escherichia coli str. K-12 substr. MG1655


Cut out the band, digest PCR products and 1454 with Nde1 + Xho1



Colony Screening

[edit]

Date: 2013-

Make miniprep and digest withNde1 + Xho1




Result intepretation/Summary

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Conclusion

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