1592 pT7A3-MBP-(3c)-Ecoli-Topo I
Cloning of 1592 pT7A3-MBP-(3c)-Ecoli-Topo I
[edit]Purpose: Cloning of 1592 pT7A3-MBP-(3c)-Ecoli-Topo I
Date: 2013-2-7---
Notebook:0
Page:
Clone ID:1592
Experimenters: Shanyi
Results
[edit]PCR
[edit]PCR from genomic DNA isolated from Escherichia coli str. K-12 substr. MG1655
USE 5ul MG1655 genomic DNA as template
Cut out the band, digest PCR products and 1514 pT7A3-MBP-(3C) with Nde1 + Xho1
Colony Screening
[edit]Date: 2013-
Make miniprep and digest withNde1 + Xho1
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Result intepretation/Summary
[edit]Conclusion
[edit]Cloning of 1592 pEC-Ecoli-Topo I
[edit]Purpose: Cloning of 1592 pEC-Ecoli-Topo I
Date: 2013-4-7---
Notebook:0
Page:
Clone ID:1592
Experimenters: Shanyi
Results
[edit]PCR
[edit]PCR from genomic DNA isolated from Escherichia coli str. K-12 substr. MG1655
USE 5ul MG1655 genomic DNA as template
Cut out the band, digest PCR products and 1454 pT7A3-MBP-(3C) with Nde1 + Xho1
Colony Screening
[edit]Date: 2013-
Make miniprep and digest with Nde1 + Xho1+BamHI
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