1567 pT7A3-T5-Exo
ClopT7A3-T7-Exoning of 1567 pT7A3-T5-Exo
[edit]Purpose: Cloning of 1567 pT7A3-T5-Exo
Date:Dec, 2012
Notebook:
Page:
Clone ID: 1567
Experimenters: shanyi
Results
[edit]Cloning
[edit]subclone from 1021 pET8XHis-T5Exo using Nco1+ Xho1 to 1279
Colony Screening
[edit]
Result intepretation/Summary
[edit]
Conclusion
[edit]protein screening
[edit]
| lane |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
9 |
10 |
| clone ID | 1696-pCold-MBP-(3C)-TEV |
1707-pCold-MBP-(3C)-TEV |
1567pT7A3-T5-Exo |
1673-pET28b-Nde1 |
1697-pCold-GST-(3c)-wt3C | |||||
| host |
Nico21DE3plysN |
BL21DE3pRare2pLysS |
Nico21DE3plysN |
Nico21DE3plysN |
Nico21DE3plysN | |||||
| induction |
0.2 mM IPTG 25 oC o/n |
0.2 mM IPTG 25 oC o/n |
0.2 mM IPTG 25 oC o/n |
0.2 mM IPTG 25 oC o/n |
0.2 mM IPTG 25 oC o/n | |||||
| purification |
5ml HisTrap HP |
5ml HisTrap HP |
Ni beads |
Ni beads |
Ni beads | |||||
| MW |
72KD/6.04 |
88KD/5.76 |
33KD/5.22 |
44KD/8.41 |
21KD/8.47 | |||||