1265 pEC-Tks

Cloning of 1265 pEC-Tks-

Purpose: Cloning of1265 pEC-Tks

Date: 2013-3-

Notebook:

Page:

Clone ID:1265

Experimenters: Shanyi

Results

cloning

Date:2013-3-

Gene synthesis 4 PCR fragments

 then use Fragment1,2,3 ,4as template Tks-1-1+Tks-4-18 as primers

digest :

	band 1,2	band 3
	PCR product	1454
enzymes	Nde1 + Kpn1

Colony Screening

Date: 2013

DIGEST WITH HindIIIshould get three bands

SEND 1265-2 TO SEQUENCE,missing one G

cloning

Date:2013-3-

PCR using 1265-2as template

use Tks-1-1 and Tks-2-10, Tks-2-11 and Tks-4-18 as primers

 then use Fragment1,2as template Tks-1-1+Tks-4-18 as primers

digest :

	band 5,6	band 4
	PCR product	1454
enzymes	Nde1 + Kpn1

Colony Screening

Date: 2013

DIGEST WITH HindIIIshould get three bands

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